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Catalog
C003276
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Alternative name
Ciliary neurotrophic factor CLIA KIT; CNTF CLIA KIT; CNTF CLIA KIT
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GeneCNTF
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Species ReactivityRabbit
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SpecificityThis assay has high sensitivity and excellent specificity for detection of Ciliary Neurotrophic Factor (CNTF).
No significant cross-reactivity or interference between Ciliary Neurotrophic Factor (CNTF) and analogues was observed.
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SamplesSerum, plasma, tissue homogenates, cell culture supernates and other biological fluids
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Detection Range4.12-3000pg/mL
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SensitivityThe minimum detectable dose of this kit is typically less than 1.93pg/mL.
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PrecisionIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ciliary Neurotrophic Factor (CNTF) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ciliary Neurotrophic Factor (CNTF) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
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Product Description specificalPrinciple of the Assay: The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated secondary antibody. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Ciliary Neurotrophic Factor (CNTF) level in the sample or standard.
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