Human MITF ELISA Kit

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  • Alternative name

    Human class E basic helix-loop-helix protein 32 ELISA Kit;Human bHLHe32 ELISA Kit;Human CMM8 ELISA Kit;Human COMMAD ELISA Kit;Human MI ELISA Kit;Human WS2 ELISA Kit;Human WS2A ELISA Kit;Human melanogenesis associated transcription factor ELISA Kit;Human microphthalmia-associated transcription factor ELISA Kit;Human microphtalmia-associated transcription factor ELISA Kit;

  • Catalog
    E024459
  • species
    Human
  • GeneMITF
  • Standard CurveHuman MITF ELISA Kit
  • Other Species Mouse Mitf ELISA KitRat Mitf ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Microphthalmia Associated Transcription Factor (MITF). No significant cross-reactivity or interference between Microphthalmia Associated Transcription Factor (MITF) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.059ng/mL.
  • Intended UseHuman MITF ELISA Kit allows for the in vitro quantitative determination of MITF , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Microphthalmia Associated Transcription Factor (MITF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Microphthalmia Associated Transcription Factor (MITF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Microphthalmia Associated Transcription Factor (MITF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Microphthalmia Associated Transcription Factor (MITF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Microphthalmia-associated transcription factor Protein information
  • Uniprot ID MITF_HUMAN
  • Uniprot AC Q9P2Y8;
  • UniGene Hs.166017; Hs.618266;
  • GeneID 4286
  • KEGG hsa:4286;
  • Human Microphthalmia-associated transcription factor Protein SEQUENCE
  • SEQUENCE 526 AA; 58795 MW; 136EBED3044C1986 CRC64;

    MQSESGIVPD FEVGEEFHEE PKTYYELKSQ PLKSSSSAEH PGASKPPISS

    SSMTSRILLR QQLMREQMQE QERREQQQKL QAAQFMQQRV PVSQTPAINV

    SVPTTLPSAT QVPMEVLKVQ THLENPTKYH IQQAQRQQVK QYLSTTLANK

    HANQVLSLPC PNQPGDHVMP PVPGSSAPNS PMAMLTLNSN CEKEGFYKFE

    EQNRAESECP GMNTHSRASC MQMDDVIDDI ISLESSYNEE ILGLMDPALQ

    MANTLPVSGN LIDLYGNQGL PPPGLTISNS CPANLPNIKR ELTACIFPTE

    SEARALAKER QKKDNHNLIE RRRRFNINDR IKELGTLIPK SNDPDMRWNK

    GTILKASVDY IRKLQREQQR AKELENRQKK LEHANRHLLL RIQELEMQAR

    AHGLSLIPST GLCSPDLVNR IIKQEPVLEN CSQDLLQHHA DLTCTTTLDL

    TDGTITFNNN LGTGTEANQA YSVPTKMGSK LEDILMDDTL SPVGVTDPLL

    SSVSPGASKT SSRRSSMSME ETEHTC

  • UCSC uc003dnz.4; human. [O75030-1];


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