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Catalog
C003029
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Alternative name
Cytochrome P450 2E1 CLIA KIT; 4-nitrophenol 2-hydroxylase (EC:1.14.13.n7) CLIA KIT; CYPIIE1 CLIA KIT; Cytochrome P450-JCYP2E1 CLIA KIT; CYP2E CLIA KIT
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GeneCYP2E1
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Species ReactivityRat
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Detection Range3.125~200ng/mL
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Sensitivity1.875ng/mL
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Product Description specificalThe micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat CYP2E1. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CYP2E1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CYP2E1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit(RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat CYP2E1. You can calculate the concentration of Rat CYP2E1 in the samples by comparing the RLU value of the samples to the standard curve.
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