Mouse Vegfa ELISA Kit

Alternative name
Mouse VEGF-A ELISA Kit;Mouse vascular permeability factor ELISA Kit;Mouse Vpf ELISA Kit;Mouse Vegf ELISA Kit;Mouse vascular endothelial growth factor A ELISA Kit;
Catalog
E072351
species
Mouse
GeneVegfa
SpecificityThis assay has high sensitivity and excellent specificity for detection of Mouse VEGFA. No significant cross-reactivity or interference between Mouse VEGFA and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.857 pg/ml
Intended UseMouse Vegfa ELISA Kit allows for the in vitro quantitative determination of Vegfa , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for VEGF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any VEGF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VEGF is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VEGF bound in the initial step. The color development is stopped and the intensity of the color is measured.
Mouse Vascular endothelial growth factor A Protein information
Uniprot ID VEGFA_MOUSE
Uniprot AC Q00731; O70123; Q66S31; Q6GT23; Q6WZL9;
UniGene Mm.282184;
GeneID 22339
KEGG mmu:22339;
Mouse Vascular endothelial growth factor A Protein SEQUENCE
SEQUENCE 214 AA; 25283 MW; B5540B51E4BB6E17 CRC64;
MNFLLSWVHW TLALLLYLHH AKWSQAAPTT EGEQKSHEVI KFMDVYQRSY
CRPIETLVDI FQEYPDEIEY IFKPSCVPLM RCAGCCNDEA LECVPTSESN
ITMQIMRIKP HQSQHIGEMS FLQHSRCECR PKKDRTKPEK KSVRGKGKGQ
KRKRKKSRFK SWSVHCEPCS ERRKHLFVQD PQTCKCSCKN TDSRCKARQL
ELNERTCRCD KPRR
UCSC uc008crr.3; mouse. [Q00731-5];

Mouse Vegfa ELISA Kit

Mouse VEGF-A ELISA Kit;Mouse vascular permeability factor ELISA Kit;Mouse Vpf ELISA Kit;Mouse Vegf ELISA Kit;Mouse vascular endothelial growth factor A ELISA Kit;

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