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Catalog
C002642
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Alternative name
Fibroblast growth factor 21 CLIA KIT; FGF21 CLIA KIT; FGF-21 CLIA KIT
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GeneFGF21
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Species ReactivityRat
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SpecificityThis assay has high sensitivity and excellent specificity for detection of FGF21. No significant cross-reactivity or interference between FGF21 and analogues was observed.
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SamplesRat serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
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Detection Range1.17-300pg/mL. The standard curve concentrations used for the CLIA's were 300pg/mL, 75pg/mL, 18.75pg/mL, 4.69pg/mL, 1.17pg/mL.
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Sensitivity0.43pg/mL.
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Product Description specificalIntended Uses: The Chemiluminescent Immunoassay kit is designed for the in vitro sensitive quantitative measurement of FGF21 in rat serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Principle of the Assay||The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific to FGF21. A competitive inhibition reaction is launched between biotin labeled FGF21 and unlabeled FGF21 (Standards or samples) with the pre-coated antibody specific to FGF21. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of FGF21 in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the FGF21 level in the sample or standard.
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