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Catalog
C002159
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Alternative name
Hepatocyte nuclear factor 1-alpha CLIA KIT; Liver-specific transcription factor LF-B1 CLIA KIT; LFB1 CLIA KIT; Transcription factor 1 CLIA KIT; TCF-1HNF1A CLIA KIT; TCF1 CLIA KIT; HNF-1-alpha CLIA KIT; HNF-1A CLIA KIT; LFB1 CLIA KIT; TCF-1 CLIA KIT
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GeneHNF1a
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Species ReactivityRat
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Detection Range0.156~10ng/mL
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Sensitivity0.094ng/mL
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Product Description specificalThe micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat HNF1a. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat HNF1a and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat HNF1a, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit(RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat HNF1a. You can calculate the concentration of Rat HNF1a in the samples by comparing the RLU value of the samples to the standard curve.
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