Human PDX1 ELISA Kit

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  • Alternative name

    Human PDX-1 ELISA Kit;Human glucose-sensitive factor ELISA Kit;Human GSF ELISA Kit;Human Insulin promoter factor 1 ELISA Kit;Human IPF-1 ELISA Kit;Human insulin upstream factor 1 ELISA Kit;Human IUF-1 ELISA Kit;Human islet/duodenum homeobox-1 ELISA Kit;Human IDX-1 ELISA Kit;Human somatostatin-transactivating factor 1 ELISA Kit;Human STF-1 ELISA Kit;Human IPF1 ELISA Kit;Human STF1 ELISA Kit;Human IUF1 ELISA Kit;Human MODY4 ELISA Kit;Human PAGEN1 ELISA Kit;Human pancreatic and duodenal homeobox 1 ELISA Kit;Human pancreas/duodenum homeobox protein 1 ELISA Kit;Human insulin promoter factor 1, homeodomain transcription factor ELISA Kit;Human pancreatic-duodenal homeobox factor 1 ELISA Kit;Human somatostatin transcription factor 1 ELISA Kit;

  • Catalog
    E028166
  • species
    Human
  • GenePDX1
  • Other Species Mouse Pdx1 ELISA KitRat Pdx1 ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity< 9.4pg/ml
  • Intended UseHuman PDX1 ELISA Kit allows for the in vitro quantitative determination of PDX1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human PDX1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human PDX1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human PDX1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human PDX1 can be calculated.
  • Human Pancreas/duodenum homeobox protein 1 Protein information
  • Uniprot ID PDX1_HUMAN
  • Uniprot AC P52945; O60594; Q5VYW2;
  • UniGene Hs.32938;
  • GeneID 3651
  • KEGG hsa:3651;
  • Human Pancreas/duodenum homeobox protein 1 Protein SEQUENCE
  • SEQUENCE 283 AA; 30771 MW; 5D48EC8D3289F72B CRC64;

    MNGEEQYYAA TQLYKDPCAF QRGPAPEFSA SPPACLYMGR QPPPPPPHPF

    PGALGALEQG SPPDISPYEV PPLADDPAVA HLHHHLPAQL ALPHPPAGPF

    PEGAEPGVLE EPNRVQLPFP WMKSTKAHAW KGQWAGGAYA AEPEENKRTR

    TAYTRAQLLE LEKEFLFNKY ISRPRRVELA VMLNLTERHI KIWFQNRRMK

    WKKEEDKKRG GGTAVGGGGV AEPEQDCAVT SGEELLALPP PPPPGGAVPP

    AAPVAAREGR LPPGLSASPQ PSSVAPRRPQ EPR

  • UCSC uc001urt.3; human.;



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