Human PDX1 ELISA Kit

Alternative name
Human PDX-1 ELISA Kit;Human glucose-sensitive factor ELISA Kit;Human GSF ELISA Kit;Human Insulin promoter factor 1 ELISA Kit;Human IPF-1 ELISA Kit;Human insulin upstream factor 1 ELISA Kit;Human IUF-1 ELISA Kit;Human islet/duodenum homeobox-1 ELISA Kit;Human IDX-1 ELISA Kit;Human somatostatin-transactivating factor 1 ELISA Kit;Human STF-1 ELISA Kit;Human IPF1 ELISA Kit;Human STF1 ELISA Kit;Human IUF1 ELISA Kit;Human MODY4 ELISA Kit;Human PAGEN1 ELISA Kit;Human pancreatic and duodenal homeobox 1 ELISA Kit;Human pancreas/duodenum homeobox protein 1 ELISA Kit;Human insulin promoter factor 1, homeodomain transcription factor ELISA Kit;Human pancreatic-duodenal homeobox factor 1 ELISA Kit;Human somatostatin transcription factor 1 ELISA Kit;
Catalog
E028166
species
Human
GenePDX1
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity< 9.4pg/ml
Intended UseHuman PDX1 ELISA Kit allows for the in vitro quantitative determination of PDX1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human PDX1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human PDX1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human PDX1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human PDX1 can be calculated.
Human Pancreas/duodenum homeobox protein 1 Protein information
Uniprot ID PDX1_HUMAN
Uniprot AC P52945; O60594; Q5VYW2;
UniGene Hs.32938;
GeneID 3651
KEGG hsa:3651;
Human Pancreas/duodenum homeobox protein 1 Protein SEQUENCE
SEQUENCE 283 AA; 30771 MW; 5D48EC8D3289F72B CRC64;
MNGEEQYYAA TQLYKDPCAF QRGPAPEFSA SPPACLYMGR QPPPPPPHPF
PGALGALEQG SPPDISPYEV PPLADDPAVA HLHHHLPAQL ALPHPPAGPF
PEGAEPGVLE EPNRVQLPFP WMKSTKAHAW KGQWAGGAYA AEPEENKRTR
TAYTRAQLLE LEKEFLFNKY ISRPRRVELA VMLNLTERHI KIWFQNRRMK
WKKEEDKKRG GGTAVGGGGV AEPEQDCAVT SGEELLALPP PPPPGGAVPP
AAPVAAREGR LPPGLSASPQ PSSVAPRRPQ EPR
UCSC uc001urt.3; human.;

Category

Human PDX1 ELISA Kit