SpecificityThis assay has high sensitivity and excellent specificity for detection of T3. No significant cross-reactivity or interference between T3 and analogues was observed.
Detection Range78.1-20,000pg/mL. The standard curve concentrations used for the CLIA's were 20,000pg/mL, 5,000pg/mL, 1,250pg/mL, 312.5pg/mL, 78.1pg/mL.
SensitivityThe minimum detectable dose of T3 is typically less than 25.9pg/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean RLU value of 20 replicates of the zero standard subtracted by their two standard deviations.
Product Description specificalPrinciple of the assay: The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific to T3. A competitive inhibition reaction is launched between biotin labeled T3 and unlabeled T3 (Standards or samples) with the pre-coated antibody specific to T3. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of T3 in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the T3 level in the sample or standard.