Human DMKN ELISA Kit

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  • Alternative name

    Human epidermis-specific secreted protein SK30/SK89 ELISA Kit;Human UNQ729 ELISA Kit;Human ZD52F10 ELISA Kit;Human dermokine ELISA Kit;

  • Catalog
    E011749
  • species
    Human
  • GeneDMKN
  • Standard CurveHuman DMKN ELISA Kit
  • Other Species Bovine DMKN ELISA KitMouse Dmkn ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Dermokine (DMKN). No significant cross-reactivity or interference between Dermokine (DMKN) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.064ng/mL.
  • Intended UseHuman DMKN ELISA Kit allows for the in vitro quantitative determination of DMKN , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dermokine (DMKN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dermokine (DMKN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dermokine (DMKN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dermokine (DMKN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Dermokine Protein information
  • Uniprot ID DMKN_HUMAN
  • Uniprot AC Q6E0U5; Q6UXC7; Q96EW8; Q9BSY6;
  • UniGene Hs.417795;
  • GeneID 93099
  • KEGG hsa:93099;
  • Human Dermokine Protein SEQUENCE
  • SEQUENCE 476 AA; 47082 MW; C412EC42221BEEC8 CRC64;

    MKFQGPLACL LLALCLGSGE AGPLQSGEES TGTNIGEALG HGLGDALSEG

    VGKAIGKEAG GAAGSKVSEA LGQGTREAVG TGVRQVPGFG VADALGNRVG

    EAAHALGNTG HEIGRQAEDV IRHGADAVRG SWQGVPGHNG AWETSGGHGI

    FGSQGGLGGQ GQGNPGGLGT PWVHGYPGNS AGSFGMNPQG APWGQGGNGG

    PPNFGTNTQG AVAQPGYGSV RASNQNEGCT NPPPSGSGGG SSNSGGGSGS

    QSGSSGSGSN GDNNNGSSSG GSSSGSSSGG SSGGSSGGSS GNSGGSRGDS

    GSESSWGSST GSSSGNHGGS GGGNGHKPGC EKPGNEARGS GESGIQNSET

    SPGMFNFDTF WKNFKSKLGF INWDAINKNQ VPPPSTRALL YFSRLWEDFK

    QNTPFLNWKA IIEGADASSL QKRAGRDDQN YNYNQHAYPT AYGGKYSVKT

    PAKGGVSPSS SASRVQPGLL QWVKFW

  • UCSC uc002nzi.5; human. [Q6E0U4-1];


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