SpecificityThis assay has high sensitivity and excellent specificity for detection of Mucin 5 Subtype B (MUC5B).
No significant cross-reactivity or interference between Mucin 5 Subtype B (MUC5B) and analogues was observed.
SamplesTissue homogenates, cell lysates, cell culture supernates and other biological fluids
SensitivityThe minimum detectable dose of this kit is typically less than 19.4pg/mL.
PrecisionIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mucin 5 Subtype B (MUC5B) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mucin 5 Subtype B (MUC5B) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Product Description specificalPrinciple of the Assay: The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated secondary antibody. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Mucin 5 Subtype B (MUC5B) level in the sample or standard.