anti-Vimentin antibody

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  • Alternative name

    FLJ36605, VIM, Vimentin antibody

  • Catalog
  • Size100μg
  • Formliquid
  • Purity≥95% as determined by SDS-PAGE
  • StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
  • ClonalityPolyclonal Antibody
  • Host Rabbit
  • Species ReactivityHuman,Mouse ,Rat
  • Applications Tested/SuitableELISA,IF,IHC,WB,FC
  • PurificationImmunogen affinity purified
  • Immunogenvimentin
  • IHCImmunohistochemistry of paraffin-embedded human tonsillitis using A009408(VIM antibody) at dilution of 1:50
  • Western BlotNIH/3T3 cells were subjected to SDS PAGE followed by western blot with A009408(VIM antibody) at dilution of 1:2000
  • Recommended dilutionWB :1:1000-1:10000;IHC:1:20-1:500;IF:1:50-1:500
  • Product Description specificalVimentin, also named as VIM, belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Monocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha (TNFA; 191160) and inhibited by the antiinflammatory cytokine IL10 (124092), suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes. As a phosphoprotein, 55-60 kDa of vimentin proteins can be observed due to the different phosphorylation level.
  • Gene nameVIM
  • locationCytoplasm,Intermediate filament
  • Uniprot IDP08670
  • Calculated M.W.55kDa
  • Observed M.W.57 kDa

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