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Alternative name
Deubiquitinating enzyme 33, hVDU1, KIAA1097, Ubiquitin thiolesterase 33, USP33, VDU1 antibody
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Catalog
A009325
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Size100μg
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Formliquid
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Purity≥95% as determined by SDS-PAGE
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StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
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ClonalityPolyclonal Antibody
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Host Rabbit
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Species ReactivityHuman,Mouse ,Rat
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Applications Tested/SuitableELISA,WB,IHC
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PurificationImmunogen affinity purified
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Immunogenubiquitin specific peptidase 33
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IHCImmunohistochemistry of paraffin-embedded human prostate cancer slide using A009325( USP33 Antibody) at dilution of 1:50
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Western BlotHEK-293 cells were subjected to SDS PAGE followed by western blot with A009325(USP33 antibody) at dilution of 1:300
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Recommended dilutionWB : 1:200-1:2000;IP:1：200-1：1000;IHC:1：20-1：200
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Product Description specificalDeubiquitinating enzyme involved in various processes such as centrosome duplication, cellular migration and beta-2 adrenergic receptor/ADRB2 recycling. Involved in regulation of centrosome duplication by mediating deubiquitination of CCP110 in S and G2/M phase, leading to stabilize CCP110 during the period which centrioles duplicate and elongate. Involved in cell migration via its interaction with intracellular domain of ROBO1, leading to regulate the Slit signaling. Plays a role in commissural axon guidance cross the ventral midline of the neural tube in a Slit-dependent manner, possibly by mediating the deubiquitination of ROBO1. Acts as a regulator of G-protein coupled receptor (GPCR) signaling by mediating the deubiquitination of beta-arrestins (ARRB1 and ARRB2) and beta-2 adrenergic receptor (ADRB2). Plays a central role in ADRB2 recycling and resensitization after prolonged agonist stimulation by constitutively binding ADRB2, mediating deubiquitination of ADRB2 and inhibiting lysosomal trafficking of ADRB2. Upon dissociation, it is probably transferred to the translocated beta- arrestins, leading to beta-arrestins deubiquitination and disengagement from ADRB2. This suggests the existence of a dynamic exchange between the ADRB2 and beta-arrestins. Deubiquitinates DIO2, thereby regulating thyroid hormone regulation. Mediates deubiquitination of both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
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Gene nameUSP33
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locationCytoplasm,Cytoskeleton,Golgi apparatus
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Uniprot IDQ8TEY7
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Calculated M.W.107kDa
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Observed M.W.103 kDa,107 kDa
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