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Alternative name
HUPF3B, hUpf3p X, MRXS14, RENT3B, UPF3B, UPF3X antibody
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Catalog
A009272
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Size100μg
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Formliquid
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Purity≥95% as determined by SDS-PAGE
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StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
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ClonalityPolyclonal Antibody
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Host Rabbit
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Species ReactivityHuman,Mouse ,Rat
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Applications Tested/SuitableELISA,WB,IHC
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PurificationImmunogen affinity purified
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ImmunogenUPF3 regulator of nonsense transcripts homolog B (yeast)
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IHCImmunohistochemistry of paraffin-embedded human testis slide using A009272( UPF3B Antibody) at dilution of 1:50
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Western BlotJurkat cells were subjected to SDS PAGE followed by western blot with A009272(UPF3B antibody) at dilution of 1:2000
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Recommended dilutionWB : 1:500-1:5000;IHC：1:20-1:200
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Product Description specificalInvolved in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons by associating with the nuclear exon junction complex (EJC) and serving as link between the EJC core and NMD machinery. Recruits UPF2 at the cytoplasmic side of the nuclear envelope and the subsequent formation of an UPF1-UPF2- UPF3 surveillance complex (including UPF1 bound to release factors at the stalled ribosome) is believed to activate NMD. In cooperation with UPF2 stimulates both ATPase and RNA helicase activities of UPF1. Binds spliced mRNA upstream of exon-exon junctions. In vitro, stimulates translation; the function is independent of association with UPF2 and components of the EJC core.
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Gene nameUPF3B
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locationCytoplasm,Nucleus
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Uniprot IDQ9BZI7
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Calculated M.W.58kDa
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Observed M.W.58 kDa
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