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Alternative name
HUpf3, HUPF3A, RENT3A, UPF3, UPF3A antibody
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Catalog
A009271
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Size100μg
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Formliquid
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Purity≥95% as determined by SDS-PAGE
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StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
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ClonalityPolyclonal Antibody
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Host Rabbit
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Species ReactivityHuman, Mouse ,Rat
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Applications Tested/SuitableELISA,WB,IHC
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PurificationImmunogen affinity purified
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ImmunogenUPF3 regulator of nonsense transcripts homolog A (yeast)
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IHCImmunohistochemistry of paraffin-embedded human testis using A009271(UPF3A antibody) at dilution of 1:50
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Western Blothuman testis tissue were subjected to SDS PAGE followed by western blot with A009271(UPF3A antibody) at dilution of 1:500
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Recommended dilutionWB : 1:500-1:5000;IHC：1:20-1:200；IF:1：20-1：200
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Product Description specificalInvolved in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons by associating with the nuclear exon junction complex (EJC) and serving as link between the EJC core and NMD machinery. Recruits UPF2 at the cytoplasmic side of the nuclear envelope and the subsequent formation of an UPF1-UPF2- UPF3 surveillance complex (including UPF1 bound to release factors at the stalled ribosome) is believed to activate NMD. However, UPF3A is shown to be only marginally active in NMD as compared to UPF3B. Binds spliced mRNA upstream of exon-exon junctions. In vitro, weakly stimulates translation.
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Gene nameUPF3A
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locationCytoplasm,Nucleus
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Uniprot IDQ9H1J1
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Calculated M.W.51kDa
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Observed M.W.55 kDa
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