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Alternative name
Ubiquitin carboxyl terminal hydrolase isozyme L3, Ubiquitin thioesterase L3, UCHL3, UCH-L3 antibody
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Catalog
A009219
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Size100μg
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Formliquid
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Purity≥95% as determined by SDS-PAGE
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StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
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ClonalityPolyclonal Antibody
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Host Rabbit
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Species ReactivityHuman,Mouse ,Rat
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Applications Tested/SuitableELISA,WB,IHC
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PurificationImmunogen affinity purified
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Immunogenubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase)
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IHCImmunohistochemistry of paraffin-embedded human pancreas cancer using A009219(UCHL3 antibody) at dilution of 1:50
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Western BlotHeLa cells were subjected to SDS PAGE followed by western blot with A009219(UCHL3 antibody) at dilution of 1:500
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Recommended dilutionWB : 1:200-1:2000;IHC：1：20-1:200;IP:1:200-1:2000
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Product Description specificalDeubiquitinating enzyme (DUB) that controls levels of cellular ubiquitin through processing of ubiquitin precursors and ubiquitinated proteins. Thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8. Has a 10-fold preference for Arg and Lys at position P3", and exhibits a preference towards 'Lys-48'-linked Ubiquitin chains. Deubiquitinates ENAC in apical compartments, thereby regulating apical membrane recycling. Indirectly increases the phosphorylation of IGFIR, AKT and FOXO1 and promotes insulin- signaling and insulin-induced adipogenesis. Required for stress- response retinal, skeletal muscle and germ cell maintenance. May be involved in working memory. Can hydrolyze UBB(+1), a mutated form of ubiquitin which is not effectively degraded by the proteasome and is associated with neurogenerative disorders.
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Gene nameUCHL3
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locationCytoplasm
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Uniprot IDP15374
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Calculated M.W.26kDa
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Observed M.W.26 kDa
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