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Alternative name
FLJ25987, Polyubiquitin B, UBB, ubiquitin, ubiquitin B antibody
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Catalog
A009197
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Size100μg
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Formliquid
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Purity≥95% as determined by SDS-PAGE
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StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
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ClonalityPolyclonal Antibody
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Host Rabbit
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Species ReactivityHuman,Mouse ,Rat
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Applications Tested/SuitableELISA,IHC,IP,WB
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PurificationImmunogen affinity purified
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Immunogenubiquitin B
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IHCImmunohistochemistry of paraffin-embedded human pancreas using A009197(ubiquitin antibody) at dilution of 1:50
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Western BlotMDA-MB-453s cells were subjected to SDS PAGE followed by western blot with A009197( ubiquitin Antibody) at dilution of 1:600
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Recommended dilutionWB : 1:200-1:2000;IP:1:200-1:1000;IHC:1:50-1:200
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Product Description specificalUbiquitin B (UBB) is a member of ubiquitin family, one of the most conserved proteins known. Ubiquitin B is required for ATP-dependent, non-lysosomal intracellular protein degradation of abnormal proteins and normal proteins with a rapid turnover. Ubiquitin B is covalently bound to proteins to be degraded, and presumably labels these proteins for degradation. Ubiquitin also binds to histone H2A in actively transcribed regions but does not cause histone H2A degradation, suggesting that ubiquitin is also involved in regulation of gene expression.When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling. This gene consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. Aberrant form of this protein has been noticed in patients with Alzheimer's and Down syndrome. Interestingly ubiquitin also becomes covalently bonded to many types of pathological inclusions which appear to be resistant to normal degradation.
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Gene nameUBB
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locationCytoplasm,Nucleus
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Uniprot IDP0CG47?
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Calculated M.W.26kDa,Observed 8kDa,15kDa，24kDa,34kDa
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Observed M.W.8 kDa, 24 kDa
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