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Alternative name
ANCR, AS, E6 AP, E6AP, E6AP ubiquitin protein ligase, EPVE6AP, FLJ26981, HPVE6A, UBE3A, ubiquitin protein ligase E3A antibody
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Catalog
A009189
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Size100μg
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Formliquid
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Purity≥95% as determined by SDS-PAGE
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StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
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ClonalityPolyclonal Antibody
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Host Rabbit
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Species ReactivityHuman,Mouse ,Rat
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Applications Tested/SuitableELISA,WB,IHC,IF,IP
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PurificationImmunogen affinity purified
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Immunogenubiquitin protein ligase E3A
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IHCImmunohistochemistry of paraffin-embedded human kidney using A009189(UBE3A antibody) at dilution of 1:50
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Western Blotmouse brain tissue were subjected to SDS PAGE followed by western blot with A009189(UBE3A antibody) at dilution of 1:1000
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Recommended dilutionWB : 1:500-1:5000;IHC:1:20-1:200；IP:1:200-1:1000;IF：1:10-1:100
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Product Description specificalE3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and transfers it to its substrates. Several substrates have been identified including the RAD23A and RAD23B, MCM7 (which is involved in DNA replication), annexin A1, the PML tumor suppressor, and the cell cycle regulator CDKN1B. Catalyzes the high-risk human papilloma virus E6-mediated ubiquitination of p53/TP53, contributing to the neoplastic progression of cells infected by these viruses. Additionally, may function as a cellular quality control ubiquitin ligase by helping the degradation of the cytoplasmic misfolded proteins. Finally, UBE3A also promotes its own degradation in vivo. Plays an important role in the regulation of the circadian clock: involved in the ubiquitination of the core clock component ARNTL/BMAL1, leading to its proteasomal degradation (PubMed:24728990).
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Gene nameUBE3A
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locationCytoplasm,Nucleus,Proteasome
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Uniprot IDQ05086
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Calculated M.W.100kDa
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Observed M.W.100 kDa
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