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Alternative name
NCE2, NEDD8 carrier protein UBE2F, NEDD8 conjugating enzyme 2, NEDD8 protein ligase UBE2F, UBE2F antibody
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Catalog
A009173
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Size100μg
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Formliquid
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Purity≥95% as determined by SDS-PAGE
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StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
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ClonalityPolyclonal Antibody
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Host Rabbit
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Species ReactivityHuman,Mouse ,Rat
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Applications Tested/SuitableELISA,WB,IHC
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PurificationImmunogen affinity purified
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Immunogenubiquitin-conjugating enzyme E2F (putative)
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IHCImmunohistochemistry of paraffin-embedded human skin using A009173(UBE2F antibody) at dilution of 1:100
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Western BlotJurkat cells were subjected to SDS PAGE followed by western blot with A009173(UBE2F antibody) at dilution of 1:500
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Recommended dilutionWB : 1:500-1:5000;IHC:1:20-1:200
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Product Description specificalAccepts the ubiquitin-like protein NEDD8 from the UBA3- NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX2, but not RBX1, suggests that the RBX2-UBE2F complex neddylates specific target proteins, such as CUL5.
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Gene nameUBE2F
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locationnucleus,ytoplasm
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Uniprot IDQ969M7
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Calculated M.W.21kDa
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Observed M.W.21 kDa
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