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Alternative name
E2 17kDa, HHR6B, HR6B, RAD6 homolog B, RAD6B, UBC2, UBE2B, Ubiquitin carrier protein B, Ubiquitin protein ligase B antibody
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Catalog
A009164
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Size100μg
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Formliquid
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Purity≥95% as determined by SDS-PAGE
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StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
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ClonalityPolyclonal Antibody
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Host Rabbit
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Species ReactivityHuman,Mouse ,Rat
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Applications Tested/SuitableELISA,IHC,WB
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PurificationImmunogen affinity purified
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Immunogenubiquitin-conjugating enzyme E2B (RAD6 homolog)
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IHCImmunohistochemistry of paraffin-embedded human ovary tumor using A009164(UBE2B antibody) at dilution of 1:10
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Western BlotL02 cells were subjected to SDS PAGE followed by western blot with A009164( UBE2B Antibody) at dilution of 1:600
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Recommended dilutionWB : 1:200-1:2000;IHC:1:10-1:100
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Product Description specificalAccepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In association with the E3 enzyme BRE1 (RNF20 and/or RNF40), it plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B at 'Lys-120' to form H2BK120ub1. H2BK120ub1 gives a specific tag for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, and is also a prerequisite for H3K4me and H3K79me formation. In vitro catalyzes 'Lys-11'-, as well as 'Lys-48'- and 'Lys-63'-linked polyubiquitination. Required for postreplication repair of UV-damaged DNA. Associates to the E3 ligase RAD18 to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys- 164'. May be involved in neurite outgrowth.
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Gene nameUBE2B
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locationCell membrane,Membrane,Nucleus
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Uniprot IDP63146
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Calculated M.W.17kDa
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Observed M.W.17 kDa
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