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Alternative name
DNA topoisomerase 1, DNA topoisomerase I, TOP1, TOPI, topoisomerase (DNA) I antibody
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Catalog
A008865
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Size100μg
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Formliquid
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Purity≥95% as determined by SDS-PAGE
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StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
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ClonalityPolyclonal Antibody
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Host Rabbit
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Species ReactivityHuman,Mouse ,Rat
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Applications Tested/SuitableELISA,WB,IHC,IP
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PurificationImmunogen affinity purified
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Immunogentopoisomerase (DNA) I
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IHCImmunohistochemistry of paraffin-embedded human cervical cancer using A008865(TOP1 antibody) at dilution of 1:50
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Western BlotL02 cells were subjected to SDS PAGE followed by western blot with A008865(TOP1 antibody) at dilution of 1:300
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Recommended dilutionWB : 1:200-1:2000
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Product Description specificalReleases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells. Involved in the circadian transcription of the core circadian clock component ARNTL/BMAL1 by altering the chromatin structure around the ROR response elements (ROREs) on the ARNTL/BMAL1 promoter.
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Gene nameTOP1
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Uniprot IDP11387
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Observed M.W.70 kDa
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