-
Alternative name
Astrin, DEEPEST, hMAP126, MAP126, SPAG5, sperm associated antigen 5 antibody
-
Catalog
A008143
-
Size100μg
-
Formliquid
-
Purity≥95% as determined by SDS-PAGE
-
StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
-
ClonalityPolyclonal Antibody
-
Host Rabbit
-
Species ReactivityHuman
-
Applications Tested/SuitableELISA,WB,IHC,IF,IP
-
PurificationImmunogen affinity purified
-
Immunogensperm associated antigen 5
-
IHCImmunohistochemistry of paraffin-embedded human testis using A008143(SPAG5 antibody) at dilution of 1:100
-
Western BlotHeLa cells were subjected to SDS PAGE followed by western blot with A008143(SPAG5 antibody) at dilution of 1:1000
-
Recommended dilutionWB : 1:1000-1:10000
-
Product Description specificalEssential component of the mitotic spindle required for normal chromosome segregation and progression into anaphase (PubMed:11724960, PubMed:12356910). Required for chromosome alignment, normal timing of sister chromatid segregation, and maintenance of spindle pole architecture (PubMed:17664331). In complex with SKAP, promotes stable microtubule-kinetochore attachments. May contribute to the regulation of separase activity. May regulate AURKA localization to mitotic spindle, but not to centrosomes and CCNB1 localization to both mitotic spindle and centrosomes (PubMed:18361916, PubMed:21402792). Involved in centriole duplication. Required for CDK5RAP2, CEP152, WDR62 and CEP63 centrosomal localization and promotes the centrosomal localization of CDK2 (PubMed:26297806). In non-mitotic cells, upon stress induction, inhibits mammalian target of rapamycin complex 1 (mTORC1) association and recruits the mTORC1 component RPTOR to stress granules (SGs), thereby preventing mTORC1 hyperactivation- induced apoptosis (PubMed:23953116). May enhance GSK3B-mediated phosphorylation of other substrates, such as MAPT/TAU (PubMed:18055457).
-
Gene nameSPAG5
-
Uniprot IDQ96R06
-
Observed M.W.126kd,140kd
-