StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
PurificationImmunogen affinity purified
Immunogensuppressor of cytokine signaling 1
IHCImmunohistochemistry of paraffin-embedded human placenta tissue slide using A008099( SOCS1 Antibody) at dilution of 1:50
Western BlotTransfected HEK-293 cells were subjected to SDS PAGE followed by western blot with A008099( SOCS1 Antibody) at dilution of 1:1000
Recommended dilutionWB : 1:500-1:5000
Product Description specificalSOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS1 is involved in negative regulation of cytokines that signal through the JAK/STAT3 pathway. Through binding to JAKs, inhibits their kinase activity. In vitro, also suppresses Tec protein- tyrosine activity. Appears to be a major regulator of signaling by interleukin 6 (IL6) and leukemia inhibitory factor (LIF). Regulates interferon-gamma mediated sensory neuron survival (By similarity). Probable substrate recognition component of an ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Seems to recognize JAK2. SOCS1 appears to be a negative regulator in IGF1R signaling pathway.