StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
Species ReactivityHuman, Mouse
PurificationImmunogen affinity purified
ImmunogenSET and MYND domain containing 2
IHCImmunohistochemistry of paraffin-embedded human heart using A008048(SMYD2 antibody) at dilution of 1:100
Western Blotmouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with A008048(SMYD2 antibody) at dilution of 1:500
Recommended dilutionWB : 1:500-1:5000
Product Description specificalProtein-lysine N-methyltransferase that methylates both histones and non-histone proteins, including p53/TP53 and RB1. Specifically methylates histone H3 'Lys-4' (H3K4me) and dimethylates histone H3 'Lys-36' (H3K36me2). Shows even higher methyltransferase activity on p53/TP53. Monomethylates 'Lys-370' of p53/TP53, leading to decreased DNA-binding activity and subsequent transcriptional regulation activity of p53/TP53. Monomethylates RB1 at 'Lys-860'.