StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
Species ReactivityHuman,Mouse ,Rat
PurificationImmunogen affinity purified
ImmunogenSMAD specific E3 ubiquitin protein ligase 1
IHCImmunohistochemistry of paraffin-embedded human colon cancer tissue slide using A008046( SMURF1 Antibody) at dilution of 1:50
Western BlotHEK-293 cells were subjected to SDS PAGE followed by western blot with A008046(SMURF1 antibody) at dilution of 1:800
Recommended dilutionWB : 1:200-1:2000
Product Description specificalE3 ubiquitin-protein ligase that acts as a negative regulator of BMP signaling pathway. Mediates ubiquitination and degradation of SMAD1 and SMAD5, 2 receptor-regulated SMADs specific for the BMP pathway. Promotes ubiquitination and subsequent proteasomal degradation of TRAF family members and RHOA. Plays a role in dendrite formation by melanocytes (PubMed:23999003).