StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
Species ReactivityHuman,Rat, Mouse
PurificationImmunogen affinity purified
Immunogenribonuclease L (2',5'-oligoisoadenylate synthetase-dependent)
IHCImmunohistochemistry of paraffin-embedded human spleen tissue slide using A007331( RNASEL Antibody) at dilution of 1:50
Western BlotRAW 264.7 cells were subjected to SDS PAGE followed by western blot with A007331( RNASEL Antibody) at dilution of 1:600
Recommended dilutionWB : 1:200-1:2000
Product Description specificalEndoribonuclease that functions in the interferon (IFN) antiviral response. In INF treated and virus infected cells, RNASEL probably mediates its antiviral effects through a combination of direct cleavage of single-stranded viral RNAs, inhibition of protein synthesis through the degradation of rRNA, induction of apoptosis, and induction of other antiviral genes. RNASEL mediated apoptosis is the result of a JNK-dependent stress- response pathway leading to cytochrome c release from mitochondria and caspase-dependent apoptosis. Therefore, activation of RNASEL could lead to elimination of virus infected cells under some circumstances. In the crosstalk between autophagy and apoptosis proposed to induce autophagy as an early stress response to small double-stranded RNA and at later stages of prolonged stress to activate caspase-dependent proteolytic cleavage of BECN1 to terminate autophagy and promote apoptosis (PubMed:26263979). Might play a central role in the regulation of mRNA turnover (PubMed:11585831).