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Alternative name
APG4B, AUTL1, KIAA0943 antibody
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Catalog
A000674
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Size100μg
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Formliquid
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Purity≥95% as determined by SDS-PAGE
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StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
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ClonalityPolyclonal Antibody
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Host Rabbit
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Species ReactivityHuman,Mouse ,Rat
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Applications Tested/SuitableELISA,WB,IHC,IP
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PurificationImmunogen affinity purified
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ImmunogenATG4 autophagy related 4 homolog B (S. cerevisiae)
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IHCImmunohistochemistry of paraffin-embedded human pancreas cancer using A000674(ATG4B antibody) at dilution of 1:50
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Western Blothuman brain tissue were subjected to SDS PAGE followed by western blot with A000674(ATG4B antibody) at dilution of 1:800
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Recommended dilutionWB : 1:200-1:2000
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Product Description specificalCysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3, GABARAPL1, GABARAPL2 and GABARAP, to reveal a C-terminal glycine. Exposure of the glycine at the C- terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Has also an activity of delipidating enzyme for the PE-conjugated forms.
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Gene nameATG4B
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Uniprot IDQ9Y4P1
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Observed M.W.44 kDa
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