StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
Species ReactivityHuman, Mouse
PurificationImmunogen affinity purified
ImmunogenATG12 autophagy related 12 homolog (S. cerevisiae)
IHCImmunohistochemistry of paraffin-embedded human prostate cancer using A000666(ATG12 antibody) at dilution of 1:50
Western Blothuman bladder tissue were subjected to SDS PAGE followed by western blot with A000666(ATG12 antibody) at dilution of 1:500
Recommended dilutionWB : 1:200-1:2000
Product Description specificalUbiquitin-like protein involved in autophagy vesicles formation. Conjugation with ATG5 through a ubiquitin-like conjugating system involving also ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. The ATG12-ATG5 conjugate also regulates negatively the innate antiviral immune response by blocking the type I IFN production pathway through direct association with RARRES3 and MAVS. Plays also a role in translation or delivery of incoming viral RNA to the translation apparatus.