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Alternative name
APG12, APG12L antibody
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Catalog
A000666
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Size100μg
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Formliquid
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Purity≥95% as determined by SDS-PAGE
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StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
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ClonalityPolyclonal Antibody
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Host Rabbit
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Species ReactivityHuman, Mouse
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Applications Tested/SuitableELISA,WB,IHC,IP
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PurificationImmunogen affinity purified
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ImmunogenATG12 autophagy related 12 homolog (S. cerevisiae)
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IHCImmunohistochemistry of paraffin-embedded human prostate cancer using A000666(ATG12 antibody) at dilution of 1:50
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Western Blothuman bladder tissue were subjected to SDS PAGE followed by western blot with A000666(ATG12 antibody) at dilution of 1:500
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Recommended dilutionWB : 1:200-1:2000
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Product Description specificalUbiquitin-like protein involved in autophagy vesicles formation. Conjugation with ATG5 through a ubiquitin-like conjugating system involving also ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. The ATG12-ATG5 conjugate also regulates negatively the innate antiviral immune response by blocking the type I IFN production pathway through direct association with RARRES3 and MAVS. Plays also a role in translation or delivery of incoming viral RNA to the translation apparatus.
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Gene nameATG12
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Uniprot IDO94817
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Observed M.W.48-50kd
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