StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
Species ReactivityHuman,Mouse ,Rat
PurificationImmunogen affinity purified
Immunogenpolymerase (RNA) III (DNA directed) polypeptide K, 12.3 kDa
IHCImmunohistochemistry of paraffin-embedded human gliomas using A006634(POLR3K antibody) at dilution of 1:100
Western BlotTransfected HEK-293 cells were subjected to SDS PAGE followed by western blot with A006634( POLR3K Antibody) at dilution of 1:1000
Recommended dilutionWB : 1:500-1:5000
Product Description specificalDNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway (By similarity).