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Alternative name
POLRF antibody
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Catalog
A006623
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Size100μg
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Formliquid
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Purity≥95% as determined by SDS-PAGE
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StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
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ClonalityPolyclonal Antibody
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Host Rabbit
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Species ReactivityHuman,Mouse ,Rat
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Applications Tested/SuitableELISA,WB,IF,IHC
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PurificationImmunogen affinity purified
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Immunogenpolymerase (RNA) II (DNA directed) polypeptide F
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IHCImmunohistochemistry of paraffin-embedded human cervical cancer tissue slide using A006623( POLR2F Antibody) at dilution of 1:200
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Western BlotHeLa cells were subjected to SDS PAGE followed by western blot with A006623(POLR2F antibody) at dilution of 1:500
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Recommended dilutionWB : 1:1000-1:10000
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Product Description specificalDNA-dependent RNA polymerases catalyze the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II, and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively. Pol II is the central component of the basal RNA polymerase II transcription machinery. Pols are composed of mobile elements that move relative to each other. In Pol II, POLR2F/RPB6 is part of the clamp element and together with parts of RPB1 and RPB2 forms a pocket to which the RPB4-RPB7 subcomplex binds (By similarity).
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Gene namePOLR2F
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Uniprot IDP61218
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Observed M.W.14kd
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