StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
Species ReactivityHuman, Mouse ,Rat
PurificationImmunogen affinity purified
Immunogenpolymerase (DNA directed) kappa
IHCImmunohistochemistry of paraffin-embedded human cervical cancer using A006613(POLK antibody) at dilution of 1:100
Western BlotHeLa cells were subjected to SDS PAGE followed by western blot with A006613(POLK antibody) at dilution of 1:500
Recommended dilutionWB : 1:500-1:5000
Product Description specificalDNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Depending on the context, it inserts the correct base, but causes frequent base transitions, transversions and frameshifts. Lacks 3'-5' proofreading exonuclease activity. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but does not have lyase activity.