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Alternative name
P62, sequestosome 1, SQSTM1, Ubiquitin binding protein p62 antibody
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Catalog
A006086
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Size100μg
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Formliquid
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Purity≥95% as determined by SDS-PAGE
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StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
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ClonalityPolyclonal Antibody
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Host Rabbit
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Species ReactivityHuman,Mouse ,Rat
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Applications Tested/SuitableELISA,WB,IHC,IP,IF,FC
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PurificationImmunogen affinity purified
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Immunogensequestosome 1
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IHCImmunohistochemistry of paraffin-embedded human liver cancer tissue slide using A006086( P62 SQSTM1 Antibody) at dilution of 1:50
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Western BlotHEK-293 cells were subjected to SDS PAGE followed by western blot with A006086(SQSTM1 antibody) at dilution of 1:1000
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Recommended dilutionWB:1:1000-1:10000;IP:1:1000-1:10000;IHC:1:20-1:200;IF:1:20-1:200
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Product Description specificalSequestosome 1 (SQSTM1/p62) is a multifunctional adaptor protein implicated in selective autophagy, cell signaling pathways, and tumorigenesis. p62 has been implicated in shuttling ubiquitinated and sometimes aggregated proteins for autophagic degradation. As a autophagy-specific substrate, p62 is degraded during the autophagic process, which makes intracellular level of p62 as a marker for autophagy flux. p62 is at the cross-roads of several signaling pathways including Ras/ Raf/ MAPK and NFκB and plays important role in cancer. p62 is a component of inclusion bodies/ protein aggregates found in human diseases, including Huntington's disease, Alzheimer's disease, Parkinson's disease in the brain, and nephropathic cystinosis in kidney (22074114, 22860231, 22714671). The molecular weight of p62 is predicted as 48/ 38 kDa, while western blot analyses using this antibody demonstrate the major band around 60-62 kDa in various tissues.
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Gene nameSQSTM1
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Uniprot IDQ13501
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Calculated M.W.48kDa,observed 62kDa
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Observed M.W.62 kDa
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