StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
Species ReactivityHuman, Mouse ,Rat
PurificationImmunogen affinity purified
ImmunogenADP-ribosylation factor-like 3
IHCImmunohistochemistry of paraffin-embedded human testis tissue slide using A000576( ARL3 Antibody) at dilution of 1:200
Western BlotHeLa cells were subjected to SDS PAGE followed by western blot with A000576(ARL3 antibody) at dilution of 1:1000
Recommended dilutionWB : 1:500-1:5000
Product Description specificalSmall GTP-binding protein which cycles between an inactive GDP-bound and an active GTP-bound form, and the rate of cycling is regulated by guanine nucleotide exchange factors (GEF) and GTPase-activating proteins (GAP). Required for normal cytokinesis and cilia signaling. Requires assistance from GTPase- activating proteins (GAPs) like RP2 and PDE6D, in order to cycle between inactive GDP-bound and active GTP-bound forms. Required for targeting proteins such as NPHP3 to the ciliary membrane by releasing myristoylated NPHP3 from UNC119B cargo adapter into the cilium. Does not act as an allosteric activator of the cholera toxin catalytic subunit.