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Alternative name
HEC, HEC1, KNTC2 antibody
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Catalog
A005599
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Size100μg
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Formliquid
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Purity≥95% as determined by SDS-PAGE
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StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
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ClonalityPolyclonal Antibody
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Host Rabbit
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Species ReactivityHuman,Mouse ,Rat
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Applications Tested/SuitableELISA,WB,IHC,IP
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PurificationImmunogen affinity purified
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ImmunogenNDC80 homolog, kinetochore complex component (S. cerevisiae)
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IHCImmunohistochemistry of paraffin-embedded human brain using A005599(NDC80 antibody) at dilution of 1:100
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Western BlotA2780 cells were subjected to SDS PAGE followed by western blot with A005599(NDC80 antibody) at dilution of 1:500
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Recommended dilutionWB : 1:500-1:5000
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Product Description specificalActs as a component of the essential kinetochore- associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity (PubMed:9315664, PubMed:12351790, PubMed:14654001, PubMed:14699129, PubMed:15062103, PubMed:15235793, PubMed:15239953, PubMed:15548592, PubMed:16732327). Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore (PubMed:15548592). The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules (PubMed:23085020).
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Gene nameNDC80
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Uniprot IDO14777
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Observed M.W.76 kDa
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