StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
Species ReactivityHuman,Mouse ,Rat
PurificationImmunogen affinity purified
ImmunogenNCK interacting protein with SH3 domain
IHCImmunohistochemistry of paraffin-embedded human breast cancer tissue slide using A005586( NCKIPSD Antibody) at dilution of 1:200 heat mediated antigen retrieved with Tris-EDTA buffer(pH9).
Western Blotmouse brain tissue were subjected to SDS PAGE followed by western blot with A005586(NCKIPSD antibody) at dilution of 1:500
Recommended dilutionWB : 1:200-1:2000
Product Description specificalHas an important role in stress fiber formation induced by active diaphanous protein homolog 1 (DRF1). Induces microspike formation, in vivo (By similarity). In vitro, stimulates N-WASP- induced ARP2/3 complex activation in the absence of CDC42 (By similarity). May play an important role in the maintenance of sarcomeres and/or in the assembly of myofibrils into sarcomeres. Implicated in regulation of actin polymerization and cell adhesion. Plays a role in angiogenesis.