IHCImmunohistochemistry of paraffin-embedded human lymphoma using A005060(MCM5 antibody) at dilution of 1:100
Western BlotJurkat cells were subjected to SDS PAGE followed by western blot with A005060(MCM5 antibody) at dilution of 1:500
Recommended dilutionWB : 1:1000-1:10000
Product Description specificalActs as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity (By similarity). Interacts with MCMBP.