-
Alternative name
DNA ligase 4, DNA ligase IV, LIG4, ligase IV, DNA, ATP dependent antibody
-
Catalog
A004774
-
Size100μg
-
Formliquid
-
Purity≥95% as determined by SDS-PAGE
-
StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
-
ClonalityPolyclonal Antibody
-
Host Rabbit
-
Species ReactivityHuman, Mouse
-
Applications Tested/SuitableELISA,WB,IHC
-
PurificationImmunogen affinity purified
-
Immunogenligase IV, DNA, ATP-dependent
-
IHCImmunohistochemistry of paraffin-embedded human prostate cancer using A004774(LIG4 antibody) at dilution of 1:100
-
Western Blotmouse testis tissue were subjected to SDS PAGE followed by western blot with A004774(LIG4 antibody) at dilution of 1:600
-
Recommended dilutionWB : 1:200-1:2000
-
Product Description specificalEfficiently joins single-strand breaks in a double- stranded polydeoxynucleotide in an ATP-dependent reaction. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA- dependent protein kinase complex DNA-PK to these DNA ends.
-
Gene nameLIG4
-
Uniprot IDP49917
-
Observed M.W.100-104 kDa
-