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Alternative name
DKFZp586H0919, LEF 1, LEF1, TCF1 alpha, TCF1ALPHA antibody
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Catalog
A004745
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Size100μg
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Formliquid
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Purity≥95% as determined by SDS-PAGE
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StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
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ClonalityPolyclonal Antibody
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Host Rabbit
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Species ReactivityHuman,Mouse ,Rat
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Applications Tested/SuitableELISA,IHC,WB,IP
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PurificationImmunogen affinity purified
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Immunogenlymphoid enhancer-binding factor 1
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IHCImmunohistochemistry of paraffin-embedded human thymus using A004745(LEF1 antibody) at dilution of 1:100
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Western BlotCOLO 320 cells were subjected to SDS PAGE followed by western blot with A004745( LEF1 Antibody) at dilution of 1:300
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Recommended dilutionWB : 1:200-1:1000
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Product Description specificalParticipates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. May play a role in hair cell differentiation and follicle morphogenesis. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 (By similarity). Isoform 3 lacks the CTNNB1 interaction domain and may be an antagonist for Wnt signaling. Isoform 5 transcriptionally activates the fibronectin promoter, binds to and represses transcription from the E-cadherin promoter in a CTNNB1- independent manner, and is involved in reducing cellular aggregation and increasing cell migration of pancreatic cancer cells. Isoform 1 transcriptionally activates MYC and CCND1 expression and enhances proliferation of pancreatic tumor cells.
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Gene nameLEF1
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Uniprot IDQ9UJU2
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Observed M.W.40-45 kDa
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