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Alternative name
LC3, LC3B, MAP1A/1BLC3, MAP1A/MAP1B LC3 B, MAP1A/MAP1B light chain 3 B, MAP1ALC3, MAP1LC3B antibody
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Catalog
A004720
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Size100μg
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Formliquid
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Purity≥95% as determined by SDS-PAGE
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StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
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ClonalityPolyclonal Antibody
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Host Rabbit
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Species ReactivityHuman, Mouse ,Rat
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Applications Tested/SuitableELISA,WB,IF,IHC,FC
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PurificationImmunogen affinity purified
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Immunogenmicrotubule-associated protein 1 light chain 3 beta
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IHCImmunohistochemistry of paraffin-embedded human heart using A004720(LC3B-Specific antibody) at dilution of 1:100
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Western Blotrat brain tissue were subjected to SDS PAGE followed by western blot with A004720( MAP1LC3B-Specific Antibody) at dilution of 1:600
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Recommended dilutionWB:1:500-1:5000;IHC:1:20-1:200;IF:1:20-1:200;FC:N/A
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Product Description specificalLC3B, also named as MAP1LC3B, MAP1A/1BLC3, belongs to the MAP1 LC3 family. It is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. In cell biology, autophagy, or autophagocytosis, is a catabolic process involving the degradation of a cell's own components through the lysosomalmachinery. It is a major mechanism by which a starving cell reallocates nutrients from unnecessary processes to more-essential processes. Two forms of LC3, called LC3-I (17-19kd) and -II(14-16kd), were produced post-translationally in various cells. LC3-I is cytosolic, whereas LC3-II is membrane bound. The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II. The amount of LC3-II is correlated with the extent of autophagosome formation. LC3-II is the first mammalian protein identified that specifically associates with autophagosome membranes. MAP1LC3 has 3 isoforms MAP1LC3A, MAP1LC3B and MAP1LC3C. MAP1LC3A and MAP1LC3C are produced by the proteolytic cleavage after the conserved C-terminal Gly residue, like their rat counterpart, MAP1LC3B does not undergo C-terminal cleavage and exists in a single modified form. This antibody is specific to LC3B.
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Gene nameMAP1LC3B
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locationCytoplasm,Cytoplasmic vesicle,Cytoskeleton,Membrane,Microtubule
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Uniprot IDQ9GZQ8
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Calculated M.W.15kDa
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Observed M.W.15kDa, 18 kDa
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