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Alternative name
RCH1, SRP1 antibody
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Catalog
A004635
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Size100μg
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Formliquid
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Purity≥95% as determined by SDS-PAGE
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StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
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ClonalityPolyclonal Antibody
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Host Rabbit
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Species ReactivityHuman,Mouse ,Rat
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Applications Tested/SuitableELISA,WB,IHC
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PurificationImmunogen affinity purified
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Immunogenkaryopherin alpha 2 (RAG cohort 1, importin alpha 1)
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IHCImmunohistochemistry of paraffin-embedded human colon cancer tissue slide using A004635( KPNA2 Antibody) at dilution of 1:200 heat mediated antigen retrieved with Tris-EDTA buffer(pH9).
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Western BlotHepG2 cells were subjected to SDS PAGE followed by western blot with A004635(KPNA2 antibody) at dilution of 1:1000
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Recommended dilutionWB : 1:500-1:5000
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Product Description specificalFunctions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran- dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus.
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Gene nameKPNA2
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Uniprot IDP52292
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Observed M.W.55-58 kDa
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