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Alternative name
Checkpoint protein HUS1, hHUS1, HUS1 antibody
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Catalog
A004082
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Size100μg
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Formliquid
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Purity≥95% as determined by SDS-PAGE
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StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
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ClonalityPolyclonal Antibody
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Host Rabbit
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Species ReactivityHuman
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Applications Tested/SuitableELISA,IHC,WB
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PurificationImmunogen affinity purified
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ImmunogenHUS1 checkpoint homolog (S. pombe)
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IHCImmunohistochemistry of paraffin-embedded human colon cancer using A004082(HUS1 antibody) at dilution of 1:50
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Western BlotBxPC-3 cells were subjected to SDS PAGE followed by western blot with A004082(HUS1 antibody) at dilution of 1:0
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Recommended dilutionWB : N/A
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Product Description specificalComponent of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase.
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Gene nameHUS1
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Uniprot IDO60921
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Observed M.W.32 kDa
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