-
Alternative name
Heat shock 70 kDa protein 1A，HSPA1A,Heat shock 70 kDa protein 1,HSP70-1,HSPA1, HSX70 antibody
-
Catalog
A004048
-
Size100μg
-
Formliquid
-
Purity≥95% as determined by SDS-PAGE
-
StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
-
ClonalityPolyclonal Antibody
-
Host Rabbit
-
Species ReactivityHuman,Mouse ,Rat
-
Applications Tested/SuitableELISA,IHC,WB,IF,FC,IP
-
PurificationImmunogen affinity purified
-
Immunogenheat shock 70kDa protein 1A
-
IHCImmunohistochemistry of paraffin-embedded human liver cancer tissue slide using A004048( HSP70 Antibody) at dilution of 1:200
-
Western BlotHeLa cells were subjected to SDS PAGE followed by western blot with A004048( HSP70 Antibody) at dilution of 1:5000
-
Recommended dilutionWB:1:1000-1:10000;IP:1:500-1:5000;IHC:1:50-1:500;IF:1:20-1:200
-
Product Description specificalHSPA1A , collectively known as HSP70 (also referred to HSP72), is a stress-inducible member of heat-shock protein 70 (HSP70) proteins which are highly conserved chaperons implicated in protein folding, protein refolding, protein transport, and protein targeting. Encoded by two closely linked, intronless and stress-inducible genes, HSPA1A and HSPA1B differ by only two amino acids and are believed to be fully interchangeable proteins. HSPA1A is a cytosol/nuclear protein able to translocate between cytoplasm and nucleus. Generally, HSPA1A is thought to be expressed in unstressed normal cells at low or undetectable levels. Expression of HSPA1A protein can be highly activated by various stressful stimuli. Significant up-regulation of HSPA1A has been found in various tumors. Recently it has been reported that HSPA1A can be constitutively expressed in selected cell types. HSP70 is a useful marker for exosome. This antibody well recognized the endogenous HSP70 protein in multiple cell lines. (21373891)
-
Gene nameHSPA1A
-
Uniprot IDP0DMV8
-
Calculated M.W.70kDa
-
Observed M.W.66-70 kDa
-