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Alternative name
BHLHE78, MOP1, PASD8 antibody
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Catalog
A003859
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Size100μg
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Formliquid
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Purity≥95% as determined by SDS-PAGE
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StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
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ClonalityPolyclonal Antibody
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Host Rabbit
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Species ReactivityHuman,Mouse ,Rat
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Applications Tested/SuitableELISA,IHC,IF,FC,WB
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PurificationImmunogen affinity purified
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Immunogenhypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
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IHCImmunohistochemistry of paraffin-embedded human thyroid cancer using A003859(HIF1A antibody) at dilution of 1:100
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Western Blotmouse small intestine tissue were subjected to SDS PAGE followed by western blot with A003859( HIF1a Antibody) at dilution of 1:300
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Recommended dilutionWB:1:200-1:1000;IHC:1:50-1:200;IF:1:10-1:100
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Product Description specificalFunctions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Activation requires recruitment of transcriptional coactivators such as CREBPB and EP300. Activity is enhanced by interaction with both, NCOA1 or NCOA2. Interaction with redox regulatory protein APEX seems to activate CTAD and potentiates activation by NCOA1 and CREBBP. Involved in the axonal distribution and transport of mitochondria in neurons during hypoxia.
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Gene nameHIF1A
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locationCytoplasm,Nucleus
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Uniprot IDQ16665
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Calculated M.W.93kDa,observed 120kDa
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Observed M.W.120 kDa
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