StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
Species ReactivityHuman,Mouse ,Rat
PurificationImmunogen affinity purified
Immunogenguanine nucleotide binding protein (G protein), beta 5
IHCImmunohistochemistry of paraffin-embedded human ovary tumor using A003543(GNB5 antibody) at dilution of 1:100
Western Blotmouse brain tissue were subjected to SDS PAGE followed by western blot with A003543(GNB5 antibody) at dilution of 1:1000
Recommended dilutionWB : 1:500-1:5000
Product Description specificalHeterotrimeric guanine nucleotide-binding proteins (G proteins) function as transducers downstream of G protein-coupled receptors (GPCRs) in numerous signaling cascades. The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state. The alpha subunit has a low GTPase activity that converts bound GTP to GDP, thereby terminating the signal. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.