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Alternative name
ABH5, ALKBH5, FLJ20308, OFOXD, OFOXD1 antibody
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Catalog
A000314
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Size100μg
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Formliquid
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Purity≥95% as determined by SDS-PAGE
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StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
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ClonalityPolyclonal Antibody
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Host Rabbit
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Species ReactivityHuman,Mouse ,Rat
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Applications Tested/SuitableELISA,WB
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PurificationImmunogen affinity purified
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ImmunogenalkB, alkylation repair homolog 5 (E. coli)
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Western BlotHeLa cells were subjected to SDS PAGE followed by western blot with A000314(ALKBH5 antibody) at dilution of 1:400
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Recommended dilutionWB :1:200-1:1000
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Product Description specificalDioxygenase that demethylates RNA by oxidative demethylation: specifically demethylates N(6)-methyladenosine (m6A) RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes (PubMed:23177736, PubMed:24778178, PubMed:24616105, PubMed:24489119). Can also demethylate N(6)- methyladenosine in single-stranded DNA (in vitro) (PubMed:24616105). Requires molecular oxygen, alpha-ketoglutarate and iron (PubMed:21264265, PubMed:23177736, PubMed:24778178, PubMed:24616105, PubMed:24489119). Demethylation of m6A mRNA affects mRNA processing and export (PubMed:23177736). Required for spermatogenesis (By similarity).
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Gene nameALKBH5
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location Nucleus
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Uniprot IDQ6P6C2
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Calculated M.W.51kDa
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Observed M.W.40-50 kDa
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