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Alternative name
ABH3, ALKBH3, DEPC 1, DEPC1, FLJ43614, PCA1, Prostate cancer antigen 1 antibody
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Catalog
A000312
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Size100μg
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Formliquid
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Purity≥95% as determined by SDS-PAGE
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StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
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ClonalityPolyclonal Antibody
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Host Rabbit
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Species ReactivityHuman, Mouse
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Applications Tested/SuitableELISA,WB,IHC
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PurificationImmunogen affinity purified
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ImmunogenalkB, alkylation repair homolog 3 (E. coli)
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IHCImmunohistochemistry of paraffin-embedded human lung cancer using A000312(ALKBH3 antibody) at dilution of 1:50
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Western Blotmouse heart tissue were subjected to SDS PAGE followed by western blot with A000312(ALKBH3 antibody) at dilution of 1:1000
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Recommended dilutionWB :1:500-1:5000;IHC:1:20-1:200
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Product Description specificalDioxygenase that mediates demethylation of DNA and RNA containing 1-methyladenosine (m1A) (PubMed:12486230, PubMed:12594517, PubMed:16174769, PubMed:26863196, PubMed:26863410). Repairs alkylated DNA containing 1- methyladenosine (m1A) and 3-methylcytosine (m3C) by oxidative demethylation (PubMed:12486230, PubMed:12594517, PubMed:16174769). Has a strong preference for single-stranded DNA (PubMed:12486230, PubMed:12594517, PubMed:16174769). Able to process alkylated m3C within double-stranded regions via its interaction with ASCC3, which promotes DNA unwinding to generate single-stranded substrate needed for ALKBH3 (PubMed:22055184). Also acts on RNA (PubMed:12594517, PubMed:16174769, PubMed:26863196, PubMed:26863410, PubMed:16858410). Demethylates N(1)- methyladenosine (m1A) RNA, an epigenetic internal modification of messenger RNAs (mRNAs) highly enriched within 5'-untranslated regions (UTRs) and in the vicinity of start codons (PubMed:26863196, PubMed:26863410). Requires molecular oxygen, alpha-ketoglutarate and iron (PubMed:22055184, PubMed:16858410).
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Gene nameALKBH3
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locationCytoplasm,Nucleus
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Uniprot IDQ96Q83
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Calculated M.W.33kDa,observed 25kDa
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Observed M.W.22-25 kDa
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