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Alternative name
RAD2 antibody
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Catalog
A003075
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Size100μg
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Formliquid
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Purity≥95% as determined by SDS-PAGE
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StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
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ClonalityPolyclonal Antibody
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Host Rabbit
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Species ReactivityHuman,Mouse ,Rat
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Applications Tested/SuitableELISA,WB,IHC
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PurificationImmunogen affinity purified
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Immunogenflap structure-specific endonuclease 1
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IHCImmunohistochemistry of paraffin-embedded human colon cancer tissue slide using A003075( FEN1 Antibody) at dilution of 1:50
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Western BlotNIH/3T3 cells were subjected to SDS PAGE followed by western blot with A003075(FEN1 antibody) at dilution of 1:500
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Recommended dilutionWB : 1:500-1:5000
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Product Description specificalStructure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double- stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.
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Gene nameFEN1
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Uniprot IDP39748
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Observed M.W.48 kDa
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