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Alternative name
FBW7 , antibody
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Catalog
A003057
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Size100μg
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Formliquid
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Purity≥95% as determined by SDS-PAGE
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StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
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ClonalityPolyclonal Antibody
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Host Rabbit
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Species ReactivityHuman, Mouse ,Rat
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Applications Tested/SuitableELISA,WB,IHC
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PurificationImmunogen affinity purified
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ImmunogenF-box and WD repeat domain containing 7
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IHCImmunohistochemistry of paraffin-embedded human skin cancer tissue slide using A003057( FBXW7 Antibody) at dilution of 1:200 heat mediated antigen retrieved with Tris-EDTA buffer(pH9).
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Western Blothuman brain tissue were subjected to SDS PAGE followed by western blot with A003057(FBXW7 antibody) at dilution of 1:600
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Recommended dilutionWB : 1:200-1:2000
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Product Description specificalSubstrate recognition component of an SCF (SKP1-CUL1-F- box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Recognizes and binds phosphorylated sites/phosphodegrons within target proteins and thereafter bring them to the SCF complex for ubiquitination (PubMed:17434132). Identified substrates include cyclin-E (CCNE1 or CCNE2), JUN, MYC, NOTCH1 released notch intracellular domain (NICD), and probably PSEN1 (PubMed:11565034, PubMed:12354302, PubMed:11585921, PubMed:15103331, PubMed:14739463, PubMed:17558397, PubMed:17873522, PubMed:22608923). Acts as a negative regulator of JNK signaling by binding to phosphorylated JUN and promoting its ubiquitination and subsequent degradation (PubMed:14739463).
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Gene nameFBXW7
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Uniprot IDQ969H0
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Observed M.W.60-65 kDa
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