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Alternative name
FBX9, VCIA1 antibody
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Catalog
A003051
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Size100μg
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Formliquid
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Purity≥95% as determined by SDS-PAGE
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StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
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ClonalityPolyclonal Antibody
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Host Rabbit
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Species ReactivityHuman, Mouse ,Rat
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Applications Tested/SuitableELISA,WB,IHC
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PurificationImmunogen affinity purified
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ImmunogenF-box protein 9
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IHCImmunohistochemistry of paraffin-embedded human prostate cancer using A003051(FBXO9 antibody) at dilution of 1:50
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Western Blothuman liver tissue were subjected to SDS PAGE followed by western blot with A003051(FBXO9 antibody) at dilution of 1:300
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Recommended dilutionWB : 1:200-1:2000
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Product Description specificalSubstrate recognition component of a SCF (SKP1-CUL1-F- box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of TTI1 and TELO2 in a CK2-dependent manner, thereby directly regulating mTOR signaling. SCF(FBXO9) recognizes and binds mTORC1-bound TTI1 and TELO2 when they are phosphorylated by CK2 following growth factor deprivation, leading to their degradation. In contrast, the SCF(FBXO9) does not mediate ubiquitination of TTI1 and TELO2 when they are part of the mTORC2 complex. As a consequence, mTORC1 is inactivated to restrain cell growth and protein translation, while mTORC2 is activated due to the relief of feedback inhibition by mTORC1.
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Gene nameFBXO9
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Uniprot IDQ9UK97
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Observed M.W.52 kDa
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