StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
Species ReactivityHuman,Mouse ,Rat
PurificationImmunogen affinity purified
Immunogenestrogen receptor 2 (ER beta)
IHCImmunohistochemistry of paraffin-embedded human cervix using A002866(ESR2 antibody) at dilution of 1:50
Western BlotNIH/3T3 cells were subjected to SDS PAGE followed by western blot with A002866( ESR2 Antibody) at dilution of 1:2000
Recommended dilutionWB : 1:1000-1:10000
Product Description specificalNuclear hormone receptor. Binds estrogens with an affinity similar to that of ESR1, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner (PubMed:20074560). Isoform beta-cx lacks ligand binding ability and has no or only very low ere binding activity resulting in the loss of ligand-dependent transactivation ability. DNA-binding by ESR1 and ESR2 is rapidly lost at 37 degrees Celsius in the absence of ligand while in the presence of 17 beta-estradiol and 4-hydroxy-tamoxifen loss in DNA-binding at elevated temperature is more gradual.