StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
Species ReactivityHuman, Mouse
PurificationImmunogen affinity purified
Immunogenelongation factor RNA polymerase II
IHCImmunohistochemistry of paraffin-embedded human ovary tumor using A002740(ELL antibody) at dilution of 1:50
Western BlotHeLa cells were subjected to SDS PAGE followed by western blot with A002740(ELL antibody) at dilution of 1:1000
Recommended dilutionWB : 1:200-1:2000
Product Description specificalElongation factor component of the super elongation complex (SEC), a complex required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA. Elongation factor component of the little elongation complex (LEC), a complex required to regulate small nuclear RNA (snRNA) gene transcription by RNA polymerase II and III (PubMed:22195968, PubMed:23932780). Specifically required for stimulating the elongation step of RNA polymerase II- and III-dependent snRNA gene transcription (PubMed:23932780). ELL also plays an early role before its assembly into in the SEC complex by stabilizing RNA polymerase II recruitment/initiation and entry into the pause site. Required to stabilize the pre-initiation complex and early elongation.